Sterilization is a process of killing all life forms of microorganisms
that exist in the sample/sample, equipment or certain environments. In the
field of bacteriology, the word sterilization is often used to describe the steps
taken to achieve the goal of eliminating or killing all life forms of
For sterilizing medical devices, it might be possible to do it yourself.
However, it can be very risky if you don’t know the procedure. It does not need
to pay a lot of costs, but it is very risky for further use. You can also ask
for medical equipment sterilization services. Of course, look for those who are
already professionals in their fields. One recommended is medical device
delivery. Not only sterilization services but in rhenus-lupprians also
provides various medical devices. Starting from the smallest to the largest.
The following are the usual methods for sterilization
1. Dry heating.
The dry heat is less effective when the temperature is high enough. To
achieve effectiveness, heating is needed to reach temperatures between 160 ° C
to 180 ° C. At this temperature will cause damage to living cells and tissue;
this is due to the occurrence of auto-oxidation so that pathogenic bacteria can
catch fire. In the dry heating system, there is air; it is already known that
air is a poor conductor of heat so sterilization through dry heating takes
quite a long time, the average time required is 45 minutes. At 160 ° C, it
takes 1 hour, while at 180 ° C it takes 30 minutes.
2. How to sterilize with radiation.
In microbiology, the light wave radiation that is widely used is
ultraviolet light, gamma or X-rays and sunlight. sunlight contains a lot of
ultraviolet light so that it can directly be used for the sterilization
process; it has long been known to people. ultraviolet light can be obtained by
using a hot cathode (thermal emission) ie into a low-pressure cathode tube
filled with mercury vapor; wavelengths produced in the process are usually in
the order of 2,500 to 2,600 Angstroms.
3. How to sterilize by heating with water vapor
The object to be sterilized is placed on a sieve plate and does not
directly hit the water underneath. Heating is done until the water boils
(estimated at 100 ° C), at a pressure of 15 lb the temperature reaches 121 ° C.
Non-spherical organisms can be killed in just 10 minutes. Many types of spores
can only die by heating 100 ° C for 30 minutes but some types of spores can
survive at that temperature for several hours. Spores that can last for 10
hours at 100 ° C can be killed in just 30 minutes if the boiling water is added
with sodium carbonate (Na2 CO3).
4. Methods of sterilization by incineration
This method is carried out on platinum equipment, chromium which will be
sterilized can be done through direct combustion on the bunzen lamp flame until
it reaches the area goes out. It’s just that in the process of direct
combustion of these equipment becomes damaged over time. Its virtue:
microorganisms will all be destroyed.
5. Methods of sterilization by filtration
Different filtration methods by heating. Sterilization by heating can
kill microorganisms but dead microorganisms remain in the material, while
sterilization by way of microorganism filtration remains dormant only separated
from the material.